Peptidylarginine deiminase (PG_1424), Porphyromonas gingivalis, recombinant

Organism: Porphyromonas gingivalis (strain ATCC BAA-308 / W83). Source: E.coli. Expression Region: 44-556aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AFQETNPPAG PVRAIAEYER SAAVLVRYPF GIPMELIKEL AKNDKVITIV ASESQKNTVI TQYTQSGVNL SNCDFIIAKT DSYWTRDYTG WFAMYDTNKV GLVDFIYNRP RPNDDEFPKY EAQYLGIEMF GMKLKQTGGN YMTDGYGSAV QSHIAYTENS SLSQAQVNQK MKDYLGITHH DVVQDPNGEY INHVDCWGKY LAPNKILIRK VPDNHPQHQA LEDMAAYFAA QTCAWGTKYE VYRALATNEQ PYTNSLILNN RVFVPVNGPA SVDNDALNVY KTAMPGYEII GVKGASGTPW LGTDALHCRT HEVADKGYLY IKHYPILGEQ AGPDYKIEAD VVSCANATIS PVQCYYRING SGSFKAADMT MESTGHYTYS FTGLNKNDKV EYYISAADNS GRKETYPFIG EPDPFKFTCM NETNTCTVTG AAKALRAWFN AGRSELAVSV SLNIAGTYRI KLYNTAGEEV AAMTKELVAG TSVFSMDVYS QAPGTYVLVV EGNGIRETMK ILK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Deiminates the guanidino group of C-terminal arginine residues on a variety of peptides, including the vasoregulatory peptide-hormone bradykinin, to yield ammonia and a citrulline residue. May promote the growth of the pathogen in the periodontal pocket by producing ammonia, ammonia having a protective effect during acidic cleaning cycles in the mouth. Reference: "Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis, peptidylarginine deiminase."McGraw W.T., Potempa J., Farley D., Travis J.Infect. Immun. 67:3248-3256(1999). Function: Deiminates the guanidino group of C-terminal arginine residues on a variety of peptides, including the vasoregulatory peptide-hormone bradykinin, to yield ammonia and a citrulline residue. May promote the growth of the pathogen in the periodontal pocket by producing ammonia, ammonia having a protective effect during acidic cleaning cycles in the mouth.