Peptidoglycan-associated lipoprotein (pal), Pasteurella multocida, recombinant

Organism: Pasteurella multocida (strain Pm70). Source: E.coli. Expression Region: 20-150aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: CGSSKKDESA GQMFGGYSVQ DLQQRYNTVY FGFDKYNIEG EYVQILDAHA AFLNATPATK VVVEGNTDER GTPEYNIALG QRRADAVKHY LSAKGVQAGQ VSTVSYGEEK PAVLGHDEAA YSKNRRAVLA Y. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Reference: "Pasteurella multocida produces a protein with homology to the P6 outer membrane protein of Haemophilus influenzae." Kasten R.W., Hansen L.M., Hinojoza J., Bieber D., Ruehl W.W., Hirsh D.C. Infect. Immun. 63:989-993(1995). Function: nan