Peptidoglycan-associated lipoprotein (pal), Biotinylated, Haemophilus influenzae, recombinant

Organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd). Source: E.coli. Expression Region: 20-153aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: CSSSNNDAAG NGAAQTFGGY SVADLQQRYN TVYFGFDKYD ITGEYVQILD AHAAYLNATP AAKVLVEGNT DERGTPEYNI ALGQRRADAV KGYLAGKGVD AGKLGTVSYG EEKPAVLGHD EAAYSKNRRA VLAY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Reference: "Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae." Deich R.A., Metcalf B.J., Finn C.W., Farley J.E., Green B.A. J. Bacteriol. 170:489-498(1988). Function: nan