Penicillin-binding protein 4 (pbpE), Bacillus subtilis, recombinant

Organism: Bacillus subtilis (strain 168). Source: E.coli. Expression Region: 1-451aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MKQNKRKHLQ TLFETLGEKH QFNGTVLAAE GGDILYHHSF GYAEMTEKRP LKTNSLFELA SLSKPFTALG IILLEEKGIL GYEDKVDRWL PGFPYQGVTI RHLLNHTSGL PDYMGWFFAN WDSHKIAVNQ DIVDMLMNEG LSGYFEPNEG WMYSNTGYVL LAVIIEKASG MSYADFIKTS IFLPAGMNET RVYNRRLSPE RIDHYAYGYV YDVHSETYVL PDELEETNYV VYLDGIQGDG TVNSVTSDLF RFDQALYQDD FISKASKESA FSPVRLNNGE TIDYGFGWVL QNSPEKGRIV SHSGGWPGYS TMMIRYIDHR KTLIYLSNKE EDTEYEQAIL KAAEHILFGQ PYDVPERPAD KKKKAIDTAI YSRYVGSYLL QDGTAAQVTT ENERLYLEIA GQLRLELFPS SETRFFLRAL SVEVEFTLGE DAAKSFILYE DGSEEEAVRT K. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probably involved in peptidoglycan modification during cortex synthesis. Reference: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997). Function: Probably involved in peptidoglycan modification during cortex synthesis.