Pectin methylesterase 8A, Ruminiclostridium thermocellum

Pectin methylesterase 8A (RtPme8A), assigned the E.C. number 3.1.1.11, is a derivative of Ruminiclostridium thermocellum. It is an enzyme that removes the methyl side chains of pectins. The recombinant RtPme8A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 8 (CE8) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Pectin methylesterases. Family: CE8. Ec Number: 3.1.1.11. Organism: Ruminiclostridium thermocellum. Architecture: CE8. Optimal pH: 8.5. Optimal Temperature: 50°C. Substrates: pectins