Pectin acetyl esterase 12B, Clostridium thermocellum

Pectin acetyl esterase 12B (CtPae12B), assigned the E.C. number 3.1.1.-, is a derivative of Clostridium thermocellum. It is an enzyme that participates in the deacetylation of pectins. The recombinant CtPae12B, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 12 (CE12) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Pectin acetyl esterases. Family: CE12. Ec Number: 3.1.1.-. Organism: Clostridium thermocellum. Architecture: CE12. Optimal pH: 7.5. Optimal Temperature: 65°C. Substrates: 4-nitrophenyl acetate