Outer membrane protein Omp38 (omp38), Acinetobacter baumannii, recombinant

Organism: Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81). Source: E.coli. Expression Region: 20-356aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ANAGVTVTPL LLGYTFQDSQ HNNGGKDGNL TNGPELQDDL FVGAALGIEL TPWLGFEAEY NQVKGDVDGA SAGAEYKQKQ INGNFYVTSD LITKNYDSKI KPYVLLGAGH YKYDFDGVNR GTRGTSEEGT LGNAGVGAFW RLNDALSLRT EARATYNADE EFWNYTALAG LNVVLGGHLK PAAPVVEVAP VEPTPVAPQP QELTEDLNME LRVFFDTNKS NIKDQYKPEI AKVAEKLSEY PNATARIEGH TDNTGPRKLN ERLSLARANS VKSALVNEYN VDASRLSTQG FAWDQPIADN KTKEGRAMNR RVFATITGSR TVVVQPGQEA AAPAAAQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Functions as a porin. Induces apoptosis in human cell lines through caspase-dependent and AIF-dependent pathways. Purified Omp38 enters host cell and localizes to the mitochondria, which presumably leads to a release of proapoptotic molecules such as cytochrome c and AIF (apoptosis-inducing factor). Binds peptidoglycan, contributes to cell wall maintenance (Probable). Reference: "Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells." Choi C.H., Lee E.Y., Lee Y.C., Park T.I., Kim H.J., Hyun S.H., Kim S.A., Lee S.K., Lee J.C. Cell. Microbiol. 7:1127-1138(2005). Function: nan