ORC1 BAH domain (human, recombinant)

ORC1, a component of the Origin Recognition Complex, is necessary for the initiation of DNA replication. It is bound in complex with ORC2, ORC3, ORC4, ORC5, and ORC6. The BromoAdjacent Homology (BAH) domain of ORC1 recognizes histone 4 dimethylated at lysine 20, which is enriched at replication origins (ORI). Chromatin immunoprecipitation and parallel sequencing of ORC1 DNA binding sites has been used to identify the ORI sites in HeLa cells. In addition to BAH mediated nucleosome recognition, ORC1 contains ATPase activity which is required for loading the minichromosome maintenance helicase complex. ORC1 levels oscillate during the cell cycle, decreasing during S phase following formation of pre-replication complexes. Mutations in ORC1 have been shown to cause Meier-Gorlin syndrome, a form of primordial dwarfism. This protein product contains the BAH domain region of ORC1.Synonyms: ORC1L, Origin Recognition Complex Subunit 1, PARC1, Replication Control Protein 1. Purity: >80%. Source: Recombinant N-terminal GST-tagged protein expressed in E. coli. Amino Acids: 2-185 (C-terminal truncation). MW: 48.2 kDa. Formulation: (Request formulation change), 20 mM Tris, pH 8.0, with 50 mM sodium chloride and 20% glycerol.