Oligogalacturonate lyase 22A, Dickeya dadantii

Oligogalacturonate lyase 22A (DdOgl22A), assigned the E.C. number 4.2.2.6, is a derivative of Dickeya dadantii. It is an enzyme that uses a beta-elimination mechanism to catalyze the conversion of saturated and unsaturated digalacturonate into monogalacturonate and 4,5-unsaturated monogalacturonate-like molecules. The recombinant DdOgl22A, purified from Escherichia coli, is a single-domain Pectate Lyase family 22 (PL22) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Oligogalacturonate lyases. Family: PL22. Ec Number: 4.2.2.6. Organism: Dickeya dadantii. Architecture: PL22. Optimal pH: 7.2. Optimal Temperature: 30°C. Substrates: oligogalacturonides