O-acyltransferase (S197A), recombinant Tropaeolum majus

Organism: Tropaeolum majus (Common nasturtium) (Indian cress). Source: in vitro E.coli expression system. Expression Region: 1-518aa(S197A). Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MAVAESSQNT TTMSGHGDSD LNNFRRRKPS SSVIEPSSSG FTSTNGVPAT GHVAENRDQD RVGAMENATG SVNLIGNGGG VVIGNEEKQV GETDIRFTYR PSFPAHRRVR ESPLSSDAIF KQSHAGLFNL CIVVLIAVNS RLIIENLMKY GWLIDTGFWF SSRSLGDWSI FMCCLTLPIF PLAAFIVEKL VQRNHIAELV AVLLHVIVST AAVLYPVIVI LTCDSVYMSG VVLMLFGCIM WLKLVSYAHT SSDIRTLAKS GYKGDAHPNS TIVSCSYDVS LKSLAYFMVA PTLCYQPSYP RSSCIRKGWV VRQFVKLIVF IGLMGFIIEQ YINPIVRNSK HPLKGDFLYA IERVLKLSVP NLYVWLCMFY SFFHLWLNIL AELLRFGDRE FYKDWWNAKT VAEYWKMWNM PVHRWMVRHL YFPCLRNGIP KEGAIIIAFL VSGAFHELCI AVPCHVFKLW AFIGIMFQVP LVLITNYLQE KFSNSMVGNM IFWFIFCILG QPMCVLLYYH DLINLKEK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: "Characterization of a putative diacylglycerol acyltransferase mRNA from Tropaeolum majus embryo." Mietkiewska E., Pedersen K., Katavic V., Taylor D.C. Submitted (JUN-2005). Function: nan