Nucleoside-specific channel-forming protein tsx (tsx), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 23-294aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AENDKPQYLS DWWHQSVNVV GSYHTRFGPQ IRNDTYLEYE AFAKKDWFDF YGYADAPVFF GGNSDAKGIW NHGSPLFMEI EPRFSIDKLT NTDLSFGPFK EWYFANNYIY DMGRNKDGRQ STWYMGLGTD IDTGLPMSLS MNVYAKYQWQ NYGAANENEW DGYRFKIKYF VPITDLWGGQ LSYIGFTNFD WGSDLGDDSG NAINGIKTRT NNSIASSHIL ALNYDHWHYS VVARYWHDGG QWNDDAELNF GNGNFNVRST GWGGYLVVGY NF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Functions as substrate-specific channel for nucleosides and deoxynucleosides. Has a greater affinity for deoxynucleosides than for nucleosides, and does not transport free bases. In addition, constitutes the receptor for colicin K and phage T6. , (Microbial infection) Serves as a receptor for CdiA-STECO31, required for adhesion between E.coli expressing CdiA-STECO31 and this strain. Reference: "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006). Function: nan