Nucleoprotein (NP), partial, Reston ebolavirus, recombinant

Organism: Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus). Source: E.coli. Expression Region: 414-739aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: RPNLGSRQDD DNEIPFPGPI SNNPDQDHLE DDPRDSRDTI IPNSAIDPED GDFENYNGYH DDEVGTAGDL VLFDLDDHED DNKAFELQDS SPQSQREIER ERLIHPPPGN NKDDNRASDN NQQSADSEEQ EGQYNRHRGP ERTTANRRLS PVHEEDTPID QGDDDPSSPP PLESDDDDAS SSQQDPDYTA VAPPAPVYRS AEAHEPPHKS SNEPAETSQL NEDPDIGQSK SMQKLGETYH HLLRTQGPFE AINYYHMMKD EPVIFSTDDG KEYTYPDSLE EAYPPWLTEK ERLDNENRYI YINNQQFFWP VMSPRDKFLA ILQHHQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. Reference: "Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes." Ikegami T., Calaor A.B., Miranda M.E., Niikura M., Saijo M., Kurane I., Yoshikawa Y., Morikawa S. Arch. Virol. 146:2021-2027(2001). Function: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).