Nucleolar GTP-binding protein 2 (GNL2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-731aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQS SLQKFQEEMD TVMKDPYKVV MKQSKLPMSL LHDRIRPHNL KVHILDTESF ETTFGPKSQR KRPNLFASDM QSLIENAEMS TESYDQGKDR DLVTEDTGVR NEAQEEIYKK GQSKRIWGEL YKVIDSSDVV VQVLDARDPM GTRSPHIETY LKKEKPWKHL IFVLNKCDLV PTWATKRWVA VLSQDYPTLA FHASLTNPFG KGAFIQLLRQ FGKLHTDKKQ ISVGFIGYPN VGKSSVINTL RSKKVCNVAP IAGETKVWQY ITLMRRIFLI DCPGVVYPSE DSETDIVLKG VVQVEKIKSP EDHIGAVLER AKPEYISKTY KIDSWENAED FLEKLAFRTG KLLKGGEPDL QTVGKMVLND WQRGRIPFFV KPPNAEPLVA PQLLPSSSLE VVPEAAQNNP GEEVTETAGE GSESIIKEET EENSHCDANT EMQQILTRVR QNFGKINVVP QFSGDDLVPV EVSDLEEELE SFSDEEEEEQ EQQRDDAEES SSEPEEENVG NDTKAVIKAL DEKIAKYQKF LDKAKAKKFS AVRISKGLSE KIFAKPEEQR KTLEEDVDDR APSKKGKKRK AQREEEQEHS NKAPRALTSK ERRRAVRQQR PKKVGVRYYE THNVKNRNRN KKKTNDSEGQ KHKRKKFRQK Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation. Reference: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012). Function: GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.