Nuclear pore complex protein Nup85 (NUP85), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-656aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-TF-tagged. Target Protein Sequence: MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS CPFIYIIRKD VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR VSKNYRSVIR ACMEEMHQVA IAAKDPANGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHPSLWQLG VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVRSIC KILAMKAVRN NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK YREFHRMYGE KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE GSLEGS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis. Reference: "Proteomic analysis of the mammalian nuclear pore complex." Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. J. Cell Biol. 158:915-927(2002). Function: nan