NHE6, Human (Na, H Exchanger 6, KIAA0267) (Control Peptide)

Control peptide for N2020-50 (antiserum) and N2020-55 (affinity purified antibody). Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. , A mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267). NHE6 is 669aa (~75kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix. NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart. Applications: Suitable for use in ELISA and antibody blocking experiments. Other applications not tested. Recommended Dilution: ELISA and antibody blocking experiments: 5-10ug control peptide per 1ug affinity pure IgG or 1ul of antiserum. Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.