NHE5, Human (Na, H Exchanger 5) (Control Peptide)

Control peptide for N2019-09 (antiserum) and N2019-11(affinity purified antibody). Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. , NHE5 (chromosome 16q22.1) is expressed in human brain, testis, spleen and skeletal muscle. It is involved in pH regulation to eliminate acids generated by active metabolism. It is a major proton extruding system driven by the inward sodium ion chemical gradient (1). Applications: Suitable for use in ELISA and antibody competition studies. Other applications not tested. Recommended Dilution: ELISA: coat ELISA plates at 1ug/ml, , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.