Neutral cholesterol ester hydrolase 1 (NCEH1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-408aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SVSDPWKLML LDATFRGAQQ VSNLIHYLGL SHHLLALNFI IVSFGKKSAW SSAQVKVTDT DFDGVEVRVF EGPPKPEEPL KRSVVYIHGG GWALASAKIR YYDELCTAMA EELNAVIVSI EYRLVPKVYF PEQIHDVVRA TKYFLKPEVL QKYMVDPGRI CISGDSAGGN LAAALGQQFT QDASLKNKLK LQALIYPVLQ ALDFNTPSYQ QNVNTPILPR YVMVKYWVDY FKGNYDFVQA MIVNNHTSLD VEEAAAVRAR LNWTSLLPAS FTKNYKPVVQ TTGNARIVQE LPQLLDARSA PLIADQAVLQ LLPKTYILTC EHDVLRDDGI MYAKRLESAG VEVTLDHFED GFHGCMIFTS WPTNFSVGIR TRNSYIKWLD QNL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration. Reference: n/a. Function: nan