Neurofilament light polypeptide (NEFL), partial, swine, recombinant

Organism: Sus scrofa (Pig). Source: E.coli. Expression Region: 89-400aa. Protein Length: Partial. Tag Info: Tag-free. Target Protein Sequence: EKAQLQDLND RFASFIERVH ELEQQNKVLE AQLLVLRQKH SEPSRFRALY EQEIRDLRLA AEDATNEKQA LQGEREGLEE TLRNLQARYE EEVLSREDAE GRLMEARKGA DEAALARAEL EKRIDSLMDE IAFLKKVHEE EIAELQAQIQ YAQISVEMDV SSKPDLSAAL KDIRAQYEKL AAKNMQNAEE WFKSRFTVLT ESAAKNTDAV RAAKDEVSES RRLLKAKTLE IEACRGMNEA LEKQLQELED KQNADISAMQ DTINKLENEL RTTKSEMARY LKEYQDLLNV KMALDIEIAA YRKLLEGEET RL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. Reference: n/a. Function: nan