NADP-dependent malic enzyme, mitochondrial (ME3),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 24-590aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: RWTPTAPAQG CHSKPGPARP VPLKKRGYDV TRNPHLNKGM AFTLEERLQL GIHGLIPPCF LSQDVQLLRI MRYYERQQSD LDKYIILMTL QDRNEKLFYR VLTSDVEKFM PIVYTPTVGL ACQHYGLTFR RPRGLFITIH DKGHLATMLN SWPEDNIKAV VVTDGERILG LGDLGCYGMG IPVGKLALYT ACGGVNPQQC LPVLLDVGTN NEELLRDPLY IGLKHQRVHG KAYDDLLDEF MQAVTDKFGI NCLIQFEDFA NANAFRLLNK YRNKYCMFND DIQGTASVAV AGILAALRIT KNKLSNHVFV FQGAGEAAMG IAHLLVMALE KEGVPKAEAT RKIWMVDSKG LIVKGRSHLN HEKEMFAQDH PEVNSLEEVV RLVKPTAIIG VAAIAGAFTE QILRDMASFH ERPIIFALSN PTSKAECTAE KCYRVTEGRG IFASGSPFKS VTLEDGKTFI PGQGNNAYVF PGVALGVIAG GIRHIPDEIF LLTAEQIAQE VSEQHLSQGR LYPPLSTIRD VSLRIAIKVL DYAYKHNLAS YYPEPKDKEA FVRSLVYTPD YDSFTLD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme." Loeber G., Maurer-Fogy I., Schwendenwein R. Biochem. J. 304:687-692(1994). Function: nan