NAD-dependent methanol dehydrogenase (mdh), Bacillus methanolicus, recombinant

Organism: Bacillus methanolicus. Source: Yeast. Expression Region: 2-381aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: TNFFIPPASV IGRGAVKEVG TRLKQIGAKK ALIVTDAFLH STGLSEEVAK NIREAGLDVA IFPKAQPDPA DTQVHEGVDV FKQENCDALV SIGGGSSHDT AKAIGLVAAN GGRINDYQGV NSVEKPVVPV VAITTTAGTG SETTSLAVIT DSARKVKMPV IDEKITPTVA IVDPELMVKK PAGLTIATGM DALSHAIEAY VAKGATPVTD AFAIQAMKLI NEYLPKAVAN GEDIEAREAM AYAQYMAGVA FNNGGLGLVH SISHQVGGVY KLQHGICNSV NMPHVCAFNL IAKTERFAHI AELLGENVSG LSTAAAAERA IVALERYNKN FGIPSGYAEM GVKEEDIELL AKNAFEDVCT QSNPRVATVQ DIAQIIKNAL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the oxidation of methanol to yield formaldehyde. It possesses a NADH-dependent formaldehyde reductase activity and cannot use NADP. Reference: "Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1." Vonck J., Arfman N., de Vries G.E., van Beeumen J., van Bruggen E.F.J., Dijkhuizen L. J. Biol. Chem. 266:3949-3954(1991). Function: Catalyzes the oxidation of methanol to yield formaldehyde. It possesses a NADH-dependent formaldehyde reductase activity and cannot use NADP.