N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-223aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal GST-tagged. Target Protein Sequence: TSEVIEDEKQ FYSKAKTYWK QIPPTVDGML GGYGHISSID INSSRKFLQR FLREGPNKTG TSCALDCGAG IGRITKRLLL PLFREVDMVD ITEDFLVQAK TYLGEEGKRV RNYFCCGLQD FTPEPDSYDV IWIQWVIGHL TDQHLAEFLR RCKGSLRPNG IIVIKDNMAQ EGVILDDVDS SVCRDLDVVR RIICSAGLSL LAEERQENLP DEIYHVYSFA LR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 µg/ml can bind human NTMT1, the EC50 of human NTMT1 protein is 3.029-5.404 µg/ml. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. Reference: n/a. Function: nan