Mucin-13(MUC13),partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 19-421aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: TNQGNSADAV TTTETATSGP TVAAADTTET NFPETASTTA NTPSFPTATS PAPPIISTHS SSTIPTPAPP IISTHSSSTI PIPTAADSES TTNVNSLATS DIITASSPND GLITMVPSET QSNNEMSPTT EDNQSSGPPT GTALLETSTL NSTGPSNPCQ DDPCADNSLC VKLHNTSFCL CLEGYYYNSS TCKKGKVFPG KISVTVSETF DPEEKHSMAY QDLHSEITSL FKDVFGTSVY GQTVILTVST SLSPRSEMRA DDKFVNVTIV TILAETTSDN EKTVTEKINK AIRSSSSNFL NYDLTLRCDY YGCNQTADDC LNGLACDCKS DLQRPNPQSP FCVASSLKCP DACNAQHKQC LIKKSGGAPE CACVPGYQED ANGNCQKCAF GYSGLDCKDK FQL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MUC13 at 2 µg/mL can bind Anti-MUC13 recombinant antibody (CSB-RA887973MA1HU). The EC50 is 2.592-2.923 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl,0.5 M NaCl,250mM Imidazole 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. Reference: Muc13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells. Williams S.J., Wreschner D.H., Tran M., Eyre H.J., Sutherland G.R., McGuckin M.A. J. Biol. Chem. 276:18327-18336 (2001). Function: nan