Microtubule-associated protein tau (Mapt), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 2-752aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AEPRQEFDTM EDQAGDYTML QDQEGDMDHG LKESPPQPPA DDGSEEPGSE TSDAKSTPTA EDVTAPLVEE RAPDKQATAQ SHTEIPEGTT AEEAGIGDTP NMEDQAAGHV TQEPQKVEIF SQSLLVEPGR REGQAPDSGI SDWTHQQVPS MSGAPLPPQG LREATHQPLG TRPEDVERSH PASELLWQES PQKEAWGKDR LGSEEEVDED ITMDESSQES PPSQASLAPG TATPQARSVS ASGVSGETTS IPGFPAEGSI PLPADFFSKV SAETQASPPE GPGTGPSEEG HEAAPEFTFH VEIKASAPKE QDLEGATVVG APAEEQKARG PSVGKGTKEA SLLEPTDKQP AAGLPGRPVS RVPQLKARVA GVSKDRTGND EKKAKTSTPS CAKTPSNRPC LSPTRPTPGS SDPLIKPSSP AVCPEPATSP KYVSSVTPRN GSPGTKQMKL KGADGKTGAK IATPRGAATP GQKGTSNATR IPAKTTPSPK TPPGSGEPPK SGERSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSASK SRLQTAPVPM PDLKNVRSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV PGGGSVHIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Promotes microtubule assbly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma mbrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Reference: Cloning of a big tau microtubule-associated protein characteristic of the peripheral nervous system.Goedert M., Spillantini M.G., Crowther R.A.Proc. Natl. Acad. Sci. U.S.A. 89:1983-1987(1992). Function: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.