MICOS complex subunit Mic60 (Immt), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 34-609aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: STSSSSGVTA GKIAGAGLLF VGGGIGGTIL YAKWDSHFRE SVEKTIPYSD KLFGMVLGSA PYTVPLPKKP IQSGPLKISS VSEVMTDSEL PMAQTQETNG DTPASAAGDP APEVEHEDTI NTECPNTDEG TSTFVTAALA KSLEDALNQT ATVTRQTITA QNAAVQAVKA HSSTLKTAMD NSEIAGEKKS AQWRTVEGAL KERRKAVDEA ADALLKAKEE LEKMKTIIED AKKREIAGAT PYITAAEEKL HSMIVDLDSV VKKVQAAQSE AKVVSQYHEL VVQARDDFRK ELDSITPDIT PGWKGMSISD LAGTLSTDDL NALIAHAHRR IDQLNRELAQ QKATEKQHIE LALERQKLEE KRAFDSAVAK ALEHHRSEIQ AEQDRKVEEV RDAMENEMRT QLRRQAAAHT DHLRDVLKVQ EQELKFEFEQ DLSEKLSEQE LEFHRRSQEQ MDNFTLDINT AYARLRGIEQ AVQSHAVAEE EARKAHQLWL SVEALKYSMK TSSAEMPTIP LGSAVEAIRV SCSDNEFTQA LTAAIPPESL TRGVYSEETL RARFYAVQKL AGRVAMIDET KNSLYQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology. Reference: "Mitoproteome plasticity of rat brown adipocytes in response to cold acclimation." Navet R., Mathy G., Douette P., Dobson R.L., Leprince P., De Pauw E., Sluse-Goffart C., Sluse F.E. J. Proteome Res. 6:25-33(2007). Function: nan