Matrix metalloproteinase-24 (Mmp24), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 42-575aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AGKPAGADAP FAGQNWLKSY GYLLPYESRA SALHSGKALQ SAVSTMQQFY GIPVTGVLDQ TTIEWMKKPR CGVPDHPHLS RRRRNKRYAL TGQKWRQKHI TYSIHNYTPK VGELDTRKAI RQAFDVWQKV TPLTFEEVPY HEIKSDRKEA DIMIFFASGF HGDSSPFDGE GGFLAHAYFP GPGIGGDTHF DSDEPWTLGN ANHDGNDLFL VAVHELGHAL GLEHSNDPSA IMAPFYQYME THNFKLPQDD LQGIQKIYGP PAEPLEPTRP LPTLPVRRIH SPSERKHERH PRPPRPPLGD RPSTPGAKPN ICDGNFNTVA LFRGEMFVFK DRWFWRLRNN RVQEGYPMQI EQFWKGLPAR IDAAYERADG RFVFFKGDKY WVFKEVTVEP GYPHSLGELG SCLPREGIDT ALRWEPVGKT YFFKGERYWR YSEERRATDP GYPKPITVWK GIPQAPQGAF ISKEGYYTYF YKGRDYWKFD NQKLSVEPGY PRNILRDWMG CKQKEVERRK ERRLPQDDVD IMVTIDDVPG SVNA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin. Reference: "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009). Function: Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence