Matrix metalloproteinase-20 (Mmp20), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 107-482aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: YRLFPGEPKW KKNILTYRIS KYTPSMSPTE VDKAIQMALH AWSTAVPLNF VRINSGEADI MISFETGDHG DSYPFDGPRG TLAHAFAPGE GLGGDTHFDN AEKWTMGTNG FNLFTVAAHE FGHALGLGHS TDPSALMYPT YKYQNPYRFH LPKDDVKGIQ ALYGPRKIFP GKPTMPHIPP HKPSIPDLCD SSSSFDAVTM LGKELLFFKD RIFWRRQVHL PTGIRPSTIT SSFPQLMSNV DAAYEVAERG IAFFFKGPHY WVTRGFHMQG PPRTIYDFGF PRHVQRIDAA VYLKEPQKTL FFVGEEYYSY DERKKKMEKD YPKNTEEEFS GVSGHIDAAV ELNGYIYFFS GRKTFKYDTE KEDVVSVVKS SSWIGC. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-, -Phe-361' site. Reference: n/a. Function: nan