L-dopachrome tautomerase (Dct), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 24-472aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: QFPRVCMTLD GVLNKECCPP LGPEATNICG FLEGRGQCAE VQTDTRPWSG PYILRNQDDR EQWPRKFFNR TCKCTGNFAG YNCGGCKFGW TGPDCNRKKP AILRRNIHSL TAQEREQFLG ALDLAKKSIH PDYVITTQHW LGLLGPNGTQ PQIANCSVYD FFVWLHYYSV RDTLLGPGRP YKAIDFSHQG PAFVTWHRYH LLWLERELQR LTGNESFALP YWNFATGKNE CDVCTDELLG AARQDDPTLI SRNSRFSTWE IVCDSLDDYN RRVTLCNGTY EGLLRRNKVG RNNEKLPTLK NVQDCLSLQK FDSPPFFQNS TFSFRNALEG FDKADGTLDS QVMNLHNLAH SFLNGTNALP HSAANDPVFV VLHSFTDAIF DEWLKRNNPS TDAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTAEQLGY NYAVDLSEEE APVWSTTLS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA) (PubMed:1537333). Involved in regulating eumelanin and phaeomelanin levels. Reference: "A second tyrosinase-related protein, TRP-2, maps to and is mutated at the mouse slaty locus."Jackson I.J., Chambers D.M., Tsukamoto K., Copeland N.G., Gilbert D.J., Jenkins N.A., Hearing V.J.EMBO J. 11:527-536(1992). Function: Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA)