Keratan sulfate Endo-N-acetylglucosaminidase 111A, Bacillus circulans

Keratan sulfate Endo-N-acetylglucosaminidase 111A (BcKsh111A), assigned the E.C. number 3.2.1.-, is a derivative of Bacillus circulans. It is an endo-beta -N-acetylglucosaminidase. The recombinant BcKsh111A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 111 (GH111) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Acetylglucosaminidases. Family: GH111. Ec Number: 3.2.1.-. Organism: Bacillus circulans. Architecture: GH111. Optimal pH: 6. Optimal Temperature: 55°C. Substrates: keratan sulfate