Kallikrein 1, Recombinant, Mouse (KLK1)

The kallikreins are a family of trypsin­like serine proteases, many of which are associated with a variety of cancers (1). Kallikrein 1 (KLK1) is also known as tissue kallikrein and urinary kallikrein. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl­bradykinin (for human KLK1) (2, 3). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Recombinant mouse KLK1 was expressed with a human CD33 signal peptide and a mouse KLK5 pro­peptide, followed by the mouse KLK1 catalytic domain (residues 25 to 261). The recombinant mouse KLK1 was purified as the latent pro­form, which is readily activated by treatment with thermolysin. Source: Recombinant protein corresponding to mouse Kallikrein 1, NS0-derived, Molecular Weight: ~40-45kD, Endotoxin: <1.0EU/1ug (LAL method). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.