Isthmin-1 (Ism1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 30-454aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: SDRQDAAAGN NNLNLESDST SETSFPLSKE APEEHQVVHQ PFPRQRFPPE TGHPSLQRDG PRSFLLDLPN FPDLSKADIN GQNPNIQVTI EVVDGPDSEA EKDQHPENKP SWSLPAPDWR AWWQRSLSLA RTNSGDQDDK YDSTSDDSNF LSVPRGWDRP APGHRTFETK EQPEYDSTDG EGDWSLWSVC SVTCGNGNQK RTRSCGYACI ATESRTCDRP NCPGIEDTFR TAATEVSLLA GSEEFNATKL FEVDMDSCER WMSCKSEFLK KYMHKVINDL PSCPCSYPTE VAYSTADIFD RIKRKDFRWK DASGPKEKLE IYKPTARYCI RSMLSLESTT LAAQHCCYGD NMQLITRGKG AGTPNLISTE FSAELHYKVD VLPWIICKGD WSRYNEARPP NNGQKCTESP SDEDYIKQFQ EAREY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as an angiogenesis inhibitor. Reference: "Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer." Pera E.M., Kim J.I., Martinez S.L., Brechner M., Li S.-Y., Wessely O., De Robertis E.M. Mech. Dev. 116:169-172(2002). Function: Acts as an angiogenesis inhibitor.