Inulin fructotransferase 91A, Nonomuraea sp.

Inulin fructotransferase 91A (NIft91A), assigned the E.C. number 4.2.2.18, is a derivative of Nonomuraea sp. It is an enzyme that produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,3'-dianhydride (DFA III) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide. The recombinant NIft91A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 91 (GH91) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Inulases. Family: GH91. Ec Number: 4.2.2.18. Organism: Nonomuraea sp. Architecture: GH91. Optimal pH: 5.5. Optimal Temperature: 65°C. Substrates: inulin