Intermembrane phospholipid transport system binding protein MlaC (mlaC), recombinant Escherichia col

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 22-211aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: ADQTNPYKLM DEAAQKTFDR LKNEQPQIRA NPDYLRTIVD QELLPYVQVK YAGALVLGQY YKSATPAQRE AYFAAFREYL KQAYGQALAM YHGQTYQIAP EQPLGDKTIV PIRVTIIDPN GRPPVRLDFQ WRKNSQTGNW QAYDMIAEGV SMITTKQNEW GTLLRTKGID GLTAQLKSIS QQKITLEEKK. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. May transfer phospholipid across the periplasmic space and deliver it to the MlaFEDB complex at the inner membrane. Reference: n/a. Function: nan