Interleukin four­induced gene 1, Recombinant, Mouse (IL4-induced protein 1, LAAO, L-amino-acid oxida

Interleukin four-induced gene 1 (IL-4I1) was first identified in mouse B cells as part of the response to IL-4, a key regulator of the immune response. Later, the enzyme was shown to be a member of the flavin-containing amino acid oxidase family and closely related to L-amino acid oxidases. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with , preference toward aromatic amino acids. Its limited expression in immune tissues, dendritic cells, and macrophages indicates that it may play an important function in the immune system. Source: A DNA sequence encoding recombinant mouse IL-4-induced gene 1 (amino residues 1 to 630, NP_034345, with a substitution Ala12Val) was expressed with a C-terminal 6X-His tag in a Chinese hamster ovary (CHO) cell line., , Molecular Mass: , The purified, secreted rmIL-4I1 has the N-terminal sequence, L21DWKAASSLN. The 616 amino acid residue rmIL-4I1 predicts a molecular mass of 69kD. By SDS-PAGE under reducing conditions the protein migrates with a molecular mass of approximately 86kD. Activity: Measured by its ability to produce hydrogen peroxide during the oxidation of phenylalanine. Endotoxin: <1.0 EU/1ug (LAL) , Storage and Stability:, 12 months from date of receipt, 20 to 70°C as supplied., 1 month, 2 to 8°C under sterile conditions after reconstitution., 3 months, 20 to 70°C under sterile conditions after reconstitution., Use a manual defrost freezer and avoid repeated freeze-thaw cycles.