Inorganic Pyrophosphatase (Yeast)

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. PPase catalyzed reaction is Mg 2+ -dependent. PPase is used for in vitro transcription (IVT) to increase RNA synthesis yield by removing the inhibitory PPi. Yeast Inorganic Pyrophosphatase is heat-sensitive and loses activity rapidly above 55 °C, avoid prolonged exposure to elevated temperatures.