Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (Hadh), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 13-314aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: SSSSSASAAA KKILIKHVTV IGGGLMGAGI AQVAAATGHT VVLVDQTEDI LAKSKKGIEE SLKRMAKKKF TENPKAGDEF VEKTLSCLST STDAASVVHS TDLVVEAIVE NLKLKNELFQ RLDKFAAEHT IFASNTSSLQ ITNIANATTR QDRFAGLHFF NPVPMMKLVE VIKTPMTSQK TFESLVDFCK TLGKHPVSCK DTPGFIVNRL LVPYLIEAVR LHERGDASKE DIDTAMKLGA GYPMGPFELL DYVGLDTTKF ILDGWHEMEP ENPLFQPSPS MNNLVAQKKL GKKTGEGFYK YK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. Reference: Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013). Function: Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.