Heme-binding protein 1 (Hebp1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 1-190aa. Protein Length: Full Length. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: MLGMIRNSLF GSVETWPWQV LSTGGKEDVS YEERACEGGK FATVEVTDKP VDEALREAMP KIMKYVGGTN DKGVGMGMTV PVSFALFPNE DGSLQKKLKV WFRIPNQFQG SPPAPSDESV KIEEREGITV YSTQFGGYAK EADYVAHATQ LRTTLEGTPA TYQGDVYYCA GYDPPMKPYG RRNE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N-methylprotoporphyrin with similar affinities. Reference: "The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP." Dias J.S., Macedo A.L., Ferreira G.C., Peterson F.C., Volkman B.F., Goodfellow B.J. J. Biol. Chem. 281:31553-31561(2006). Function: nan