Group XV phospholipase A2 (Pla2g15), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 34-412aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AQRHPPVVLV PGDLGNQLEA KLDKPKVVHY LCSKKTDSYF TLWLNLELLL PVIIDCWIDN IRLVYNRTSR ATQFPDGVDV RVPGFGETFS MEFLDPSKRN VGSYFYTMVE SLVGWGYTRG EDVRGAPYDW RRAPNENGPY FLALREMIEE MYQMYGGPVV LVAHSMGNVY MLYFLQRQPQ VWKDKYIHAF VSLGAPWGGV AKTLRVLASG DNNRIPVIGP LKIREQQRSA VSTSWLLPYN HTWSHEKVFV YTPTTNYTLR DYHRFFRDIG FEDGWFMRQD TEGLVEAMTP PGVELHCLYG TGVPTPNSFY YESFPDRDPK ICFGDGDGTV NLESVLQCQA WQSRQEHRVS LQELPGSEHI EMLANATTLA YLKRVLLEP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine. Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen. Reference: "The measurement of lysosomal phospholipase A2 activity in plasma." Abe A., Kelly R., Shayman J.A. J. Lipid Res. 51:2464-2470(2010). Function: Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid