Glypican-6 (GPC6), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 24-529aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: DVKARSCGEV RQAYGAKGFS LADIPYQEIA GEHLRICPQE YTCCTTEMED KLSQQSKLEF ENLVEETSHF VRTTFVSRHK KFDEFFRELL ENAEKSLNDM FVRTYGMLYM QNSEVFQDLF TELKRYYTGG NVNLEEMLND FWARLLERMF QLINPQYHFS EDYLECVSKY TDQLKPFGDV PRKLKIQVTR AFIAARTFVQ GLTVGREVAN RVSKVSPTPG CIRALMKMLY CPYCRGLPTV RPCNNYCLNV MKGCLANQAD LDTEWNLFID AMLLVAERLE GPFNIESVMD PIDVKISEAI MNMQENSMQV SAKVFQGCGQ PKPAPALRSA RSAPENFNTR FRPYNPEERP TTAAGTSLDR LVTDIKEKLK LSKKVWSALP YTICKDESVT AGTSNEEECW NGHSKARYLP EIMNDGLTNQ INNPEVDVDI TRPDTFIRQQ IMALRVMTNK LKNAYNGNDV NFQDTSDESS GSGSGSGCMD DVCPTEFEFV TTEAPAVDPD RREVDS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling. Reference: "NFAT promotes carcinoma invasive migration through glypican-6." Yiu G.K., Kaunisto A., Chin Y.R., Toker A. Biochem. J. 440:157-166(2011). Function: nan