Glycosyltransferase-like protein LARGE1 (LARGE), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 32-756aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GSFEDGKPVS LSPLESQAHS PRYTASSQRE RESLEVRMRE VEEENRALRR QLSLAQGRAP SHRRGNHSKT YSMEEGTGDS ENLRAGIVAG NSSECGQQPV VEKCETIHVA IVCAGYNASR DVVTLVKSVL FHRRNPLHFH LIADSIAEQI LATLFQTWMV PAVRVDFYNA DELKSEVSWI PNKHYSGIYG LMKLVLTKTL PANLERVIVL DTDITFATDI AELWAVFHKF KGQQVLGLVE NQSDWYLGNL WKNHRPWPAL GRGYNTGVIL LLLDKLRKMK WEQMWRLTAE RELMGMLSTS LADQDIFNAV IKQNPFLVYQ LPCFWNVQLS DHTRSEQCYR DVSDLKVIHW NSPKKLRVKN KHVEFFRNLY LTFLEYDGNL LRRELFGCPS EADVNSENLQ KQLSELDEDD LCYEFRRERF TVHRTHLYFL HYEYEPAADS TDVTLVAQLS MDRLQMLEAI CKHWEGPISL ALYLSDAEAQ QFLRYAQGSE VLMSRHNVGY HIVYKEGQFY PVNLLRNVAM KHISTPYMFL SDIDFLPMYG LYEYLRKSVI QLDLANTKKA MIVPAFETLR YRLSFPKSKA ELLSMLDMGT LFTFRYHVWT KGHAPTNFAK WRTATTPYRV EWEADFEPYV VVRRDCPEYD RRFVGFGWNK VAHIMELDVQ EYEFIVLPNA YMIHMPHAPS FDITKFRSNK QYRICLKTLK EEFQQDMSRR YGFAALKYLT AENNS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Bifunctional glycosyltransferase with both alpha-1,3-xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the maturation of alpha-dystroglycan (DAG1) by glycosylation leading to DAG1 binding to laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Requires the phosphorylation of core M3 (O-mannosyl trisaccharide) by POMK to elongate the glucuronyl-beta-1,4-xylose-beta disaccharide primer. Plays a key role in skeletal muscle function and regeneration. Reference: n/a. Function: nan