Glucuronyl esterase 15A, Clostridium cellobioparum

Glucuronyl esterase 15A (CcGe15A), assigned the E.C. number 3.1.1.-, is a derivative of Clostridium cellobioparum. It catalyzes the hydrolysis of ester bonds, specifically those linking 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. The recombinant CcGe15A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 15 (CE15) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Glucuronyl esterases. Family: CE15. Ec Number: 3.1.1.-. Organism: Clostridium cellobioparum. Architecture: CE15. Optimal pH: 6. Optimal Temperature: 30°C. Substrates: glucuronyl crosslinks between hemicelluloses and lignin