Glial Fibrillary Acidic Protein (GFAP), human

Protein (GFAP), as a member of the cytoskeletal protein family, is the principal 8-9nm intermediate filament in mature astrocytes of the central nervous system (CNS). GFAP is a monomeric molecule with a molecular mass between 40 and 53kD and an isoelectric point between 5.7 and 5.8. GFAP is a highly brain-specific protein that is not found outside the CNS. Findings have shown that while GFAP is released into the blood very soon after traumatic brain injury (TBI) based on its severity, GFAP is not released after even multiple traumae in the absence of brain injury. In the CNS, following injury, either as a result of trauma, disease, genetic disorders, or chemical insult, astrocytes become reactive and respond in a typical manner, termed astrogliosis. Astrogliosis is characterized by the rapid synthesis of GFAP. GFAP normally increases with age and there is a wide variation in the collection and processing of human brain tissue. Thanks to its high brain specificity and early release from the CNS after TBI, GFAP might be a suitable marker for early diagnostics. Molecular Weight: ~46kD(major two bands). GFAP preparations were represented by several protein bands 43-49kD. The major two bands appear in the region of the 46kD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.