Gamma-hemolysin component C (hlgC), Staphylococcus aureus, recombinant

Organism: Staphylococcus aureus (strain N315). Source: E.coli. Expression Region: 30-315aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: ANDTEDIGKG SDIEIIKRTE DKTSNKWGVT QNIQFDFVKD KKYNKDALIL KMQGFISSRT TYYNYKKTNH VKAMRWPFQY NIGLKTNDKY VSLINYLPKN KIESTNVSQT LGYNIGGNFQ SAPSLGGNGS FNYSKSISYT QQNYVSEVEQ QNSKSVLWGV KANSFATESG QKSAFDSDLF VGYKPHSKDP RDYFVPDSEL PPLVQSGFNP SFIATVSHEK GSSDTSEFEI TYGRNMDVTH AIKRSTHYGN SYLDGHRVHN AFVNRNYTVK YEVNWKTHEI KVKGQN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity. Reference: "Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. Hiramatsu K. Lancet 357:1225-1240(2001). Function: Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity (By similarity).