G-protein coupled receptor 20 (GPR20)-VLPs (Active), recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 1-358aa. Protein Length: Full Length. Tag Info: C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions). Target Protein Sequence: MPSVSPAGPS AGAVPNATAV TTVRTNASGL EVPLFHLFAR LDEELHGTFP GLWLALMAVH GAIFLAGLVL NGLALYVFCC RTRAKTPSVI YTINLVVTDL LVGLSLPTRF AVYYGARGCL RCAFPHVLGY FLNMHCSILF LTCICVDRYL AIVRPEGSRR CRQPACARAV CAFVWLAAGA VTLSVLGVTG SRPCCRVFAL TVLEFLLPLL VISVFTGRIM CALSRPGLLH QGRQRRVRAM QLLLTVLIIF LVCFTPFHAR QVAVALWPDM PHHTSLVVYH VAVTLSSLNS CMDPIVYCFV TSGFQATVRG LFGQHGEREP SSGDVVSMHR SSKGSGRHHI LSAGPHALTQ ALANGPEA. Purity: The purity information is not available for VLPs proteins. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GPR20 at 10 µg/mL can bind Anti-GPR20 recombinant antibody(CSB-RA860774MA1HU). The EC50 is 2.431 - 3.239 ng/mL.The VLPs (CSB-MP3838) is negative control. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant). Relevance: Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP. Reference: Selective inhibition of heterotrimeric Gs signaling. Targeting the receptor-G protein interface using a peptide minigene encoding the Galpha(s) carboxyl terminus. Feldman D.S., Zamah A.M., Pierce K.L., Miller W.E., Kelly F., Rapacciuolo A., Rockman H.A., Koch W.J., Luttrell L.M. J Biol Chem 277:28631-28640 (2002). Function: nan