Feruloyl esterase 1A, Ruminococcus albus

Feruloyl esterase 1A (RaFae1A), assigned the E.C. number 3.1.1.73, is a derivative of Ruminococcus albus. It is an enzyme that hydrolyzes the ferulate groups involved in the crosslinking of hemicelluloses to lignin. The recombinant RaFae1A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 1 (CE1) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Feruloyl esterases. Family: CE1. Ec Number: 3.1.1.73. Organism: Ruminococcus albus. Architecture: CE1. Optimal pH: 7. Optimal Temperature: 37°C. Substrates: ferulate crosslinks between xylans and lignin