Fatty Acid Desaturase 2 (human recombinant)

Fatty acid desaturase 2 (FADS2), also known as Delta6-desaturase, is a 444-amino acid transmembrane protein encoded by FADS2. This product is the N-terminal cytoplasmic domain of FADS2, corresponding to residues 2-131 of the full-length sequence. It catalyzes the rate-limiting step in the conversion of the omega-6 and omega-3 precursors linoleic acid (Cay-90150, Cay-21909) and alpha-linolenic acid (Cay-90210) to their respective long-chain polyunsaturated fatty acids (LC-PUFAs), inserting a cis double bond at position 6 of the fatty acid chain to convert linoleic acid to gamma-linolenic acid (Cay-90220) and alpha-linolenic acid to stearidonic acid (Cay-90320), respectively. Following secondary desaturation by FADS1 (Cay-25827) and chain elongation, FADS2 catalyzes desaturation of 24-carbon LC-PUFA intermediates. FADS2 also catalyzes the desaturation of palmitic acid (Cay-10006627) to sapienic acid in human sebaceous glands and in various cancer cell lines that express FADS2. Hepatic FADS2 protein levels and mRNA expression are increased in mice with high-fat diet-induced obesity and non-alcoholic steatohepatitis (NASH). FADS2 knockout (fads2-/-) mice are infertile, resistant to obesity, and have altered subcellular membrane diacylglycerol composition which leads to dysregulated post-translational processing of SREBP1c, a key transcription factor in the regulation of lipid metabolism.Synonyms: Acyl-CoA 6-Desaturase, D6D, DES6, EC 1.14.19.3, FADS2, FADSD6, Delta6 Fatty Acid Desaturase, Linoleoyl-CoA Desaturase (Delta6-Desaturase)-Like 2, LLCDL2, SLL0262, TU13. Purity: >95% estimated by SDS-PAGE. Source: Recombinant N-terminal His-tagged FADS2 N-terminal domain purified from E. coli. Amino Acids: 2-131. MW: 16.99 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol. UniProt Accession #: O95864.