F-box/WD repeat-containing protein 4 (FBXW4), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-412aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MAAAAGEEEE EEEAARESAA RPAAGPALWR LPEELLLLIC SYLDMRALGR LAQVCRWLRR FTSCDLLWRR IARASLNSGF TRLGTDLMTS VPVKERVKVS QNWRLGRCRE GILLKWRCSQ MPWMQLEDDS LYISQANFIL AYQFRPDGAS LNRRPLGVFA GHDEDVCHFV LANSHIVSAG GDGKIGIHKI HSTFTVKYSA HEQEVNCVDC KGGIIVSGSR DRTAKVWPLA SGRLGQCLHT IQTEDRVWSI AISPLLSSFV TGTACCGHFS PLRIWDLNSG QLMTHLGSDF PPGAGVLDVM YESPFTLLSC GYDTYVRYWD LRTSVRKCVM EWEEPHDSTL YCLQTDGNHL LATGSSYYGV VRLWDRRQRA CLHAFPLTST PLSSPVYCLR LTTKHLYAAL SYNLHVLDFQ NP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling. Reference: "cDNA cloning and expression analysis of new members of the mammalian F-box protein family."Ilyin G.P., Rialland M., Pigeon C., Guguen-Guillouzo C.Genomics 67:40-47(2000). Function: Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.