Elastin (Eln), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 266-443aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: PLGYPIKAPK LPGGYGLPYT NGKLPYGVAG AGGKAGYPTG TGVGSQAAAA AAKAAKYGAG GAGVLPGVGG GGIPGGAGAI PGIGGIAGAG TPAAAAAAKA AAKAAKYGAA GGLVPGGPGV RLPGAGIPGV GGIPGVGGIP GVGGPGIGGP GIVGGPGAVS PAAAAKAAAK AAKYGARG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Reference: "Elastin is an essential determinant of arterial morphogenesis." Li D.Y., Brooke B., Davis E.C., Mecham R.P., Sorensen L.K., Boak B.B., Eichwald E., Keating M.T. Nature 393:276-280(1998). Function: nan