Elastase (lasB), Pseudomonas aeruginosa, recombinant

Organism: Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228). Source: E.coli. Expression Region: 198-498aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: AEAGGPGGNQ KIGKYTYGSD YGPLIVNDRC EMDDGNVITV DMNSSTDDSK TTPFRFACPT NTYKQVNGAY SPLNDAHFFG GVVFKLYRDW FGTSPLTHKL YMKVHYGRSV ENAYWDGTAM LFGDGATMFY PLVSLDVAAH EVSHGFTEQN SGLIYRGQSG GMNEAFSDMA GEAAEFYMRG KNDFLIGYDI KKGSGALRYM DQPSRDGRSI DNASQYYNGI DVHHSSGVYN RAFYLLANSP GWDTRKAFEV FVDANRYYWT ATSNYNSGAC GVIRSAQNRN YSAADVTRAF STVGVTCPSA L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase (PubMed:11533066). Autocatalyses processing of its pro-peptide (PubMed:9642203, PubMed:1744034). Processes the pro-peptide of pro-chitin-binding protein (cbpD) (PubMed:9642203). Involved in the pathogenesis of P.aeruginosa infections. Reference: "Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene."Bever R.A., Iglewski B.H.J. Bacteriol. 170:4309-4314(1988). Function: Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase