E3 ubiquitin-protein ligase pellino homolog 3 (PELI3), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-469aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MVLEGNPEVG SPRTSDLQHR GNKGSCVLSS PGEDAQPGEE PIKYGELIVL GCCEEGGEET EAQRGEVTGP RAHSCYNGCL ASGDKGRRRS RLALSRRSHA NGVKPDVMHH ISTPLVSKAL SNRGQHSISY TLSRSHSVIV EYTHDSDTDM FQIGRSTENM IDFVVTDTSP GGGAAEGPSA QSTISRYACR ILCDRRPPYT ARIYAAGFDA SSNIFLGERA AKWRTPDGLM DGLTTNGVLV MHPAGGFSED SAPGVWREIS VCGNVYTLRD SRSAQQRGKL VENESNVLQD GSLIDLCGAT LLWRTPAGLL RAPTLKQLEA QRQEANAARP QCPVGLSTLA FPSPARGRTA PDKQQPWVYV RCGHVHGYHG WGCRRERGPQ ERECPLCRLV GPYVPLWLGQ EAGLCLDPGP PSHAFAPCGH VCSEKTARYW AQTPLPHGTH AFHAACPFCG AWLTGEHGCV RLIFQGPLD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation. Reference: "Pellino3 is a novel upstream regulator of p38 MAPK and activates CREB in a p38-dependent manner." Butler M.P., Hanly J.A., Moynagh P.N. J. Biol. Chem. 280:27759-27768(2005). Function: nan