Dystrophin (Dmd), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 1-240aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MLWWEEVEDC YEREDVQKKT FTKWINAQFS KFGKQHIDNL FSDLQDGKRL LDLLEGLTGQ KLPKEKGSTR VHALNNVNKA LQVLQKNNVD LVNIGSTDIV DGNHKLTLGL IWNIILHWQV KNVMKTIMAG LQQTNSEKIL LSWVRESTRN YPQVNVLNFT SSWSDGLALN ALIHSHRPDL FDWNSVVSQQ SATQRLEHAF NIAKCQLGIE KLLDPEDVAT TYPDKKSILM YITSLFQVLP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. Reference: "Dystrophin splice variants are distinctly localized in the hippocampus." Hazai D., Halasy K., Mornet D., Hajos F., Jancsik V. Acta Biol Hung 57:141-146(2006) . Function: nan